Windows 2000 does have DirectX 9.0c support, and most video drivers for Windows XP also work under Windows 2000. So it might work.
That being said, it's not listed as a supported OS, so if you end up having problems with the game you may be out of luck support-wise.
Edit: What are your other system specs? If you're running Windows 2000 you may have an older system and may want to make sure that your other system components are up to the task.
Edited, Apr 30th 2007 12:45pm by Aldous